Bork was born in Monroe, Michigan, and relocated to Batesville, Arkansas, to teach studio and art history courses at Lyon College, where he currently serves as Professor of Art. He earned his Master of Fine Arts from Indiana University and his Bachelor of Fine Arts from the University of Michigan. 

His work has been exhibited at the Czong Institute for Contemporary Art in South Korea; Manifest Gallery in Cincinnati, Ohio; the Torpedo Art Center in Alexandria, Virginia; at the University of Southern Mississippi Museum of Art in Hattiesburg, Mississippi; at the International Print Biennial of Duoro Portugal; and the Hunterdon Museum of Art in New Jersey. In 2016, Bork won the prestigious Visual Arts Fellowship for Painting awarded by the Arkansas Arts Council.

Bork has participated in artist residencies at the Mark Rothko Art Center in Daugavpils, Latvia, Iatrus Residency in Veliko Tarnovo, Bulgaria, The Franz Masereel Centre in Kasterlee, Belgium, The MIRA artist residency in Martignano, Italy, Wilson College in Chambersburg, Pennsylvania, and at the Vermont Studio Center in Johnson City, Vermont. His work appeared in New American Paintings: Southern Issue no. 148. 

Bork is married to artist Carly Dahl, the executive director of the Batesville Area Arts Council, and has two children: Paige and Aiden Bork, who are both pursuing art careers.

Dustyn Bork is represented by Cole Pratt Gallery in New Orleans